Composite

Part:BBa_K3054027:Design

Designed by: Dharmik Hitesh Patel   Group: iGEM19_Chalmers-Gothenburg   (2019-10-20)


bphD + pTEF1/pCIT2 + bphK


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2288
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2288
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2393
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2288
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2288
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1102


Design Notes

Biobricks joined by gibson assembly. Hence need to be scarless



Source

BBa_K3054012 + BBa_K3054004 + BBa_K3054013

References